My work is based on tannase enzyme. And the assay was estimated on Gallic acid. In the chemistry of tannase it says Tannase fully hydrolysed tannic acid to form glucose and gallic acid through 2, 3, 4, and 6,-tetragalloyl glucose and two kinds of monogalloyl glucose and free gallic acid. This is evident from the fact is same and detected the same products in the hydrolysate of 1,2,3,4,6,-pentagalloylglucose and that gallic acid of methyl.m.digallate during the degradation pathway. Hence in that case whether gallic acid sample peak showned in 4.7ppm. Is it considerable a result compare with standard?
you have to change standard as well as d2o experiment i will help you
I doubt that you have gallic acid in your sample . The sample spectrum has two CH that are coupled (I guess the spectrum was run at 400 MHz). Do these peaks belong to your molecule? The peak at 4.7ppm is water .. you also have tiny peaks can't they be from the molecule you are looking for?? you have no aromatic proton at least detectable
Maybe you can try a watersuppression (to reduce the waterpeak at 4.7ppm) or a 13C experiment. But I also doubt, that you have gallic acid in you sample.
Thank you Muriel..I also done with the same sample in other experiment and got some minute amount of gallic acid. may be the quantity is less and not detected properly. Gallic is a byproduct of tannase enzyme. may be organism was not degraded properly.
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