10 December 2013 1 525 Report

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Probability of occupational allergic asthma in a patient with adult onset asthma?

What is the probability of allergic occupational astma in a patient with adult onset asthma, work related asthmatic complaints and a clear sensitization to work related allergens (laboratory...

19 November 2023 3,552 0 View

How do I send a paper?

I wish to publish how do I do so?

13 April 2023 7,688 2 View

How to adjust/control baseline outcome variable score in mixed effect model?

I am doing a mixed effect linear model using Stata and need your advice. The study is an RCT with 4 groups and 3 time points. We would like to test a group X time interaction to see the change of...

28 February 2023 107 2 View

What types of cells do you see, indicative of morphology?

I have isolated cells from murine adipose tissue from 4 month old mice and am growing them in mesencult. I know that MSCs include many cell types but I would like to focus on adipose derived stem...

01 August 2022 7,762 0 View

How can I isolate proteins from cell cultures, which contain a high amount of dead cells?

Hi everyone, I have some cell cultures of brain tumors, which contain a high amount of dead cells after detaching them with accutase (only 30% living). I tried to isolate proteins and performed a...

03 January 2022 616 3 View

How to calculate storage capacity of graphite anode?

C=(nNAe)/3.6M this is the formula for Energy storage capacity of Anode. where n is number of metal atoms adsorbed per mole of doped graphene [how this will be calculated]? Na- Avogadro's...

22 August 2021 4,143 1 View

How to digest animal tissues by use of acid?

I am investigating microplastics in a marine mollusc and would like to obtain an initial particle count by digesting the animal tissue. I am looking into procedures and reaching out to authors...

03 May 2021 2,945 5 View

How to prepare challenge microorganisms in order to obtain at not more than 100 CFU as per United States Pharmacopoeia (USP)?

In routine, I need those inoculum with a small number (NMT 100 CFU) to conduct growth promotion test. However, the inoculum preparation has not been validated so I need some references to perform...

20 February 2020 612 5 View

Experience with cheap disc-filters for medium sterilization?

I have to sterilize an MS medium containing sucrose. For reasons I cannot autoclave it and are therefore looking at 0.2 micron filter sterilization. The standard Whatman filters seem very...

19 August 2019 8,748 0 View

What is the optimal section thickness to look at the dendritic branching/morphology?

Hi everybody, I would like to exploring if manipulation of a specific gene of interest in female mice PFC alters dendritic branching/morphology. I have never done this before and was wondering...

01 April 2019 5,242 1 View

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Why wait for a doctor's visit when you can become the guardian of your child's health today?

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