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More Tia Chata's questions See All

What types of cells do you see, indicative of morphology?

I have isolated cells from murine adipose tissue from 4 month old mice and am growing them in mesencult. I know that MSCs include many cell types but I would like to focus on adipose derived stem...

01 August 2022 7,762 0 View

How can I isolate proteins from cell cultures, which contain a high amount of dead cells?

Hi everyone, I have some cell cultures of brain tumors, which contain a high amount of dead cells after detaching them with accutase (only 30% living). I tried to isolate proteins and performed a...

03 January 2022 616 3 View

How to digest animal tissues by use of acid?

I am investigating microplastics in a marine mollusc and would like to obtain an initial particle count by digesting the animal tissue. I am looking into procedures and reaching out to authors...

03 May 2021 2,945 5 View

How to prepare challenge microorganisms in order to obtain at not more than 100 CFU as per United States Pharmacopoeia (USP)?

In routine, I need those inoculum with a small number (NMT 100 CFU) to conduct growth promotion test. However, the inoculum preparation has not been validated so I need some references to perform...

20 February 2020 612 5 View

Does anyone know the average hippocampal volume of a mouse, specifically the 5xFAD mouse model of AD?

I am doing stereology to examine plaques in varying ages and genders and I wanted to also look into hippocampal volume (which the program I'm using uses the Cavalieri method to determine the...

10 November 2016 6,908 1 View

Does anyone have any suggestions on troubleshooting for transferring with western blot?

The problem that I'm having is not getting a heavy Ponceau stain OR detecting bands after I complete Western blot (addition of primary, secondary and chemi-luminescence) after transferring onto a...

23 February 2016 5,556 9 View

Any suggestions on Pacific salmon conservation methods ?

I am doing some work into conservation methods and how this can help with the population genetics of stocks of pacific salmon but the main conservation method i can find is hatcheries, i was...

28 November 2015 1,797 4 View

My raw264.7 cells are not differentiating into osteoclasts using abcam's RANKL -ab129136. Has anyone used this or has any recommendations for diff.?

Before purchasing abcam's RANKL (ab129136), I contacted the company as to whether a crosslinking antibody was necessary because I didn't want to use one. They assured me that I did not need one....

23 November 2014 612 9 View

Do we assume roles according to our feasibility in the real world or do our media role models affect us?

Gender construction and role identification is dependent on a media gender model.

19 March 2014 5,220 3 View

Does anyone know how to develop a thick aerobic biofilm on stainless steel which is tightly in static system?

I tried to develop Pseudomonas putida biofilm on stainless steel surface but it was easy to remove from the substratum.

13 February 2014 1,834 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Why is the Protocol for Preventing Postpartum Hemorrhage (https://pubmed.ncbi.nlm.nih.gov/36113283/ ) not being widely adopted?

If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...

05 August 2024 8,895 0 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

Publish a Protocol?

Hi, please suggest some journals where I can publish a observational study protocol.

27 July 2024 2,519 0 View

Please introduce me the latest published articles on the structure and properties of metal nanocomposites?

26 July 2024 2,200 3 View

Looking for articles that provide guidance on doing Thematic Analysis (Braun & Clarke) as a team?

Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...

23 July 2024 7,819 5 View

How can I find my published papers' total read, citation or any other scores?

21 July 2024 9,802 3 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

Wolfgang R. Dick

ResearchGate is not a publisher, you cannot publish a paper here. It is a platform for papers published in journals, or to present unpublished texts, data etc. See https://explore.researchgate.net/display/support/How+to+add+research for instructions how to add published or unpublished research.

Tia Chata

Thanks Wolfgang.