Is there any website providing free e books??

More Zinah Ghazi's questions See All

What is the best database concerned with genetic of breast cancer?

Can anyone help me by sending a link of useful online database for breast cancer and all genes that related with this disease.

11 December 2013 8,592 5 View

Why is the long arm (q) and the short one (p) in the chromosomes؟

Acronym for any words?

11 December 2013 3,186 12 View

How many methods can be used nowaday to detect SNPs

I just need to review all SNPs detection methods in one recent reference. Can anyone help please?

11 December 2013 4,193 2 View

What is the essential difference between mutation and polymorphism?

I'm looking to know when the variation is deemed mutation or polymorphism and why? In the case of polymorphism, it may be associated with disease so that variation should be called mutation or not?

11 December 2013 9,081 16 View

What is the difference between "technique" and "method" in molecular biology?

For example, is PCR-RFLP a technique or a method? And DNA sequencing?

11 December 2013 2,736 11 View

I don't get a homozygous genotype in the PCR-RFLP technique, is this common?

When i use PCR-RFLP technique to detect SNPs in cancer patient i just have wild- type and heterozygous genotypes but never find any homozygous in all patient and control cases and am wondering...

11 December 2013 325 19 View

Why aren't there any filter and freezing tools on design of Databases?

I'm wondering why there are no filter and freezing tools on design of Databases. I am still fledgling with bioinformatic databases and I do a copy to all the data and pass on Excel program, to...

11 December 2013 6,432 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View