Some times when sequencing on an ion torrent machine, there is a barcode that takes up a lot of data space, causing the sample data to be insufficient for analysis. But still on this Chip sequencing, re-process the chip: wash the chip, then denature again with primer sequencing and wash the chip again and add the sequencing enzyme and then run the sequencing again, then the No barcode is almost reduced. . Is there any way to reduce the No barcode at the beginning of sequencing after loading the denatured sample onto the sequencing chip?