Meysam u can go for bioautoradiography. Use TLC plates for the same.

Chk ths one @meysam

Thanks @royaha for you kindness.

But this method is even more complicated than spectrophotometry.

You can observe colour change even in visible light.

Check out the following link for non-spectrometric methods for antioxidant assay, however such methods are more complex and cumbersome

https://www.omicsonline.org/methods-for-total-antioxidant-activity-determination-a-review-2161-1009.1000106.php?aid=3538

Dear Meysam Shahrashoub,

an advantageous alternative to the spectrophotometry is the chemiluminometry, especially the method of photosensitized chemiluminescence firstly described 1986 (s. file “PCL summary.pdf”), and realized in the device Photochem® (s. files “Antioxidant capacity methods.pdf”, “PCL-NASA.pdf” and “CL-book-PCL.pdf”). The actual version of the device is currently offered by the enterprise Oxidaq UG (Berlin, Germany). The PCL method is marked through sensitivity, frugality and rapidity. To its important features belongs the possibility of the analysis of the mixture of water soluble antioxidants in biological fluids. For example, in blood plasma one can determine the shares of urate, ascorbate, bilirubin and the antiradical ability of proteins ARAP as a parameter of their oxidative modification/damage (files “Main components.jpg” and “PCL-ARAP.pdf”). Plant extracts can be investigated in the systems for measuring of water- and lipid-soluble antioxidants (file “Garlic.pdf”), the selective determination of vitamin C is also possible (file “Potato.jpg”). The results of comparative measurements of blood plasma with some drinks and also of grape components are shown in the files “Comparisons.jpg” and “Grapes.jpg”.

Regards,

I. Popov

Determination peroxide Value by Titration

I think spectrophotometric method is the most easiest method why are you looking for another method..

By titration