Is there any way of confirming whether a fungus has sporulated without making a tease/tape mount??

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Where can I procure Galleria mellonella (wax moths) from in India?

I plan to use Galleria mellonella (wax moths) as insect models for studying infection. Can someone help me in finding out from where i could get them ?

08 September 2018 3,158 2 View

Do we need to follow CPCSEA guidelines when using insect models for our research?

Since insects are invertebrates what are the guidelines for handling them in research in India ?

08 September 2018 1,472 1 View

How do i check the pH of glassware ?

I wish to check the pH of glassware which have been washed with alkaline soap. How do i proceed ?

02 March 2017 231 0 View

Can I copy paste the image generated by the geography tool in the LANL HIV database ?

Can I use the map generated by geography tool of the HIV Database at the https://www.hiv.lanl.gov ?

11 December 2016 9,147 1 View

Why cant we use Bst polymerase(used for LAMP) in place of taq polymerase for PCR ?

I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?

10 November 2016 8,969 5 View

How can I prepeare 1 % aqueous Phenol red solution ?

How can I prepare a 1 % aqueous Phenol red solution ?

10 November 2016 2,024 1 View

Do people tend to think that handsome and beautiful people are more intelligent & sincere students ?

I have a gut feeling that handsome or beautiful people are over appreciated while the inverse happens for ugly people, am I correct ?

05 June 2015 9,949 1 View

C. neoformans are found on Eucalyptus trees. Is it that they multiply on these trees or are deposited on it by birds like pigeons carrying them?

Do the C. neoformans multiply on these trees or they are just found on them ?

03 April 2015 3,497 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How can parents ensure their drinking water is free from harmful contaminants?

27 July 2024 8,722 6 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Masonry Interaction Module ABAQUS?

I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...

16 July 2024 5,047 0 View

How can we simulate(with FEM) the shape of Mobius strip using Abaqus Learning edition?

Aim is to simulate the shape of Mobius strip (an elastic ribbon) in simple way ( using Abaqus Learning edition). In literature, Mobius strip is modeled as Kirchhoff Rod / Cosserat plate. But, such...

15 July 2024 4,388 1 View