I want to target the serotonin neurons in dorsal raphe nucleus. So I ask if there is any virus promoter that enables me to target them instead of using SERT::Cre.
Unfortunately, there is none that I know of. Also, SERT:Cre might not be the ideal like. Look into ePet-Cre.
Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...
03 July 2024 3,892 1 View
I use MATLAB to define SAP Model using API function but when trying to define time history direct integration case, the non-linear parameters change from the default which affect the results and...
04 June 2024 2,369 1 View
I need it uopn a reviewer's request to make a 500ns MD
07 February 2024 7,667 3 View
Graduation projects animal biotechnology plant biotechnology microbial biotechnology bioinformatics
04 December 2023 3,530 9 View
I am performing qRT-PCR for zebrafish different organs and tissues using sybergreen and elongation factor 1-alpha elfa as a reference control. However, there is variation in the expression levels...
29 November 2023 9,309 2 View
or even the Effect of Planning on Tax Evasion. Thanks in advance,,
10 September 2023 2,941 3 View
I did CHIP qPCR for transcription factors on a promoter, I got fold enrichments (compared to control IgG) as 2.54, 2.75, 3.45 and 21 for diffferent transciption factors in different sites. Can...
28 August 2023 4,797 0 View
hello, Currently, I have MRI data results for Wistar rats, examined to confirm the induction of multiple sclerosis. However, i don't know how to interpret these images! I would very much...
29 June 2023 9,285 4 View
I use a HMC5883L-type electronic compass connected to Arduino and I got the code programming that gives me the angle of regression but the problem is that when I tested it I found that it showed...
23 June 2023 5,088 0 View
Free Valproic acid
05 June 2023 3,378 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Serotonin (5-hydroxytryptamine, 5-HT) is predominantly known as a neurotransmitter in the central nervous system (CNS), where it plays a crucial role in mood regulation, gastrointestinal motility,...
20 July 2024 3,925 5 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View