Is there any technique I can use to count the number of mitochondria in a cell or cell population?

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David Pla-Martín

Hi, you can do it following different ways:

1. WB, using ratio between mitochondrial protein and loading control (TOM20/Actin i.e)

2. Fluorescence microscopy. stain mitochondria and relate fluorescence intensity to cell area.

3. Flow cytometry: using Mitotracker Green you can achieve the mitochondrial content. This dye is independent of membrane potential so, it is not affected by mitochondrial dysfunction.

Good luck

Avinash Sharma

Thank you David, I'll see if I can use any of the method you mentioned above.

German Sabio Thanks for your suggestion I will think about it.