David Pla-Martín

6 Questions 15 Answers 0 Followers

Questions related from David Pla-Martín

How to analyze properly images from microscopy?

Hello! I have a question regarding the quantification of data obtained from microscopy. It is clear that if you take several images from the same organ/tissue, you can not consider one picture as...

21 November 2016 7,448 3 View

Which is a good Tissue Cell Lysis Buffer for Luminescent ATP assay?

Hello everybody. I would like to do measurements of ATP in pieces of liver from a KO mouse. I was wondering if anyone of you know a good buffer to solubilize liver pieces and then proceed with a...

05 February 2015 9,510 6 View

Can anyone suggest why Fura-2 AM is sometimes located in mitochondria?

That probe is expected to measure cytosolic Ca2+ movements but I found it several times in mitochondria.

27 March 2014 7,154 5 View

How to nucleofect SH-SY5Y?

I am trying to transfect SH-SY5Y cells with two plasmids with a high efficient method. I have decided to use Lonza nucleofector in order to obtain better results than using FuGene or...

05 February 2014 2,393 2 View

Does anyone know how to quench Fura-2 AM fluorescence?

I am recording changes in cytosolic calcium using Fura-2 AM in cells previously transfected with a GFP tagged protein. In order to obtain fluorescence background added by GFP I am trying to quench...

30 April 2013 2,324 12 View

How do you explain that the same sample gives different results on gene expression analysis by qPCR?

I have been using cDNA from 1ug of total RNA isolated with trizol. Also taking care with RNAse

22 March 2013 2,844 2 View