16 Questions 20 Answers 0 Followers
Questions related from Avinash Sharma
Hello everyone! I am planning an experiment where I would like to check ubiquitin transfer using confocal microscopy; thus, I intend to add a fluorophore tag to the ubiquitin molecule. I read a...
13 August 2023 7,510 1 View
Please see the attached image for the reference. I have been seeing these cells in the Arabidopsis thaliana seed (post germination) whenever I go for microscopy. The cells are attached to seed...
28 March 2023 3,098 4 View
Hi everyone, I work with Arabidopsis thaliana. I want to know what is the best strategy to strore plant saplings after the treatment to preserve the quality of RNA (at least for a week). 1. Is it...
04 February 2023 5,455 4 View
As my protein levels appears to be varying in different cell types and different layers and localization (cytoplasm/nucelus) of the root tip of Arabidopsis (in the background of Wild type and...
31 January 2023 1,216 3 View
Hello everyone! My problem is that unfortunately I am getting a good Ct value in NTC (NFW). I have tried three different primer sets (Target of three different genes) with NFW from different...
03 November 2022 7,503 9 View
So I have always used Hygromycin/Kanamycin selection for Arabidopsis but this is my first time I am using methotrexate for selection of T0 transformed plants. Plants with hygromycin resistance I...
13 December 2021 8,134 1 View
Hi everyone! I have a few lines of a particular gene of which the earlier (T2 ratio) are not known. I want to do antibiotic selection of these seeds and I want to know which plants of next...
23 May 2021 3,944 3 View
Hello everyone! I am thinking to amplify T-DNA insertion region using LBb1.3 (left T-DNA border) and RP (gene specific Reverse Primer) against the genomic DNA of one of the T-DNA insertion mutant...
12 March 2020 1,401 3 View
I performed a PCR mediated deletion of my vector which was carrying an unwanted gene (Chey 183 bp). I designed two primers with desired restriction sites in order to clone any gene in place of...
09 August 2016 4,080 4 View
I have expressed a protein of C. jejuni in E.coli M15 cells using pQE30 vector. My protein carries a N-terminal hexa his tag. I have purified my recombinant protein using Ni-NTA columns and I have...
07 August 2016 6,337 7 View
I want to study in-vitro host-pathogen interaction and subsequent immune response. In following experiment I would like to have two different cell lines in one plate say epithelial cell line and...
29 December 2015 8,116 4 View
I've got isolated strains of C. jejuni and I want to culture them, If anyone have prior experience with Campylobacter jejuni, please suggest me which media will be best to use. As there are many...
12 August 2015 5,587 13 View
Hi, I've transformed my DH5 alpha E.coli cells with pDrive vector with my GOI. After that I plated them on ampicillin agar plate. I got good number of transformed cells (as I am expecting). There...
07 August 2015 3,069 10 View
I'm going to start working with C.jejuni, as I read so many papers I found people have used so many different media for culturing C.jejuni. I want to know which media will be best for culturing...
14 June 2015 8,731 7 View
I have been trying bFGF and Forskolin protocol to differentiate Mesenchymal Stem Cells into neuronal lineage cells using a 35 mm dish but I found my cells get overcrowded within the duration of...
08 December 2014 6,671 6 View
I'm thinking to start a project where I have to check the number of mitochondria present in a cell population after certain physiological condition.
08 December 2014 626 3 View
