Hello Is there any standard Database for enzyme reaction time? for example how to know the time required to convert hypoxanthine to uric acid by xanthine oxidase? how fast is it? Thanks
kcat or enzyme specific activity is what you need. But as such data are highly dependent on the reaction condition I don't think any database has been thought...
Yeah, that's a tough one. As Dominique says turnover rates and specificity constants are so dependent on conditions it is incorrect to talk about a single value for these for an enzyme. Try BRENDA http://www.brenda-enzymes.info/ or one of the other big enzyme databases for kinetic constant's. The best advice is to think of the physiologically relevant conditions under which your enzyme works and compare the database/literature conditions to see if they vary a lot and dependent on what parameters i.e. pH, osmolarity etc