Can you please tell me how to obtain Km value or Michaelis-Menten plot for enzyme activity. In my case i would like to calculate Km value for adenosine deaminase using electrochemical methods (cyclic voltammetry)
You can calculate Michaelis-Menten constant (Km) by using Lineweaver–Burk equataion. Article Efficient electrocatalytic oxidation and selective determina...
From the slope and the intercept of a double reciprocal plot of the current and concentration plot, Km can be calculated.