In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

No title

Head-space Shimadzu GC-17A gas chromatography (Shimadzu Europa GmbH, Duisburg, Germany) was performed by using a flame ionization detector for the quantitative determination of sevoflurane loading...

02 January 2020 983 1 View

How many of you find the obscurity and face difficulty in assigning ASA physicaal status?

The American Society of Anesthesiologists physical status classification has been modified a few times to increase the uniformity. The recent amendment was done in 2014 which has also incorporated...

09 October 2019 7,136 6 View

If a diabetic patient having RBS < 180 mg%, scheduled for non-cardiac surgery in 1-2 days; is HbA1c required? if yes, why (Plz guide with evidence)?

As an anesthesiologist and also perioperative management, is more concerned with the level of blood sugar at the time or during perioperative time. If that time level is ok, then, will it have an...

19 November 2017 951 7 View

Is it more suitable to use a analgesic or anesthetic drug to avoid postoperative pain? Which API should be chosen?

The formulation is meant to give a prolonged release locally and is meant to be coated over an implant to provide pain relief caused due to the same.

10 July 2017 9,032 4 View

Intra-aneurysmal hemodynamics

26 June 2017 1,776 2 View

How can I calculate LOD and LOQ in HPLC-MS/MS?

Hi, Dear All, I got some results of my master thesis (atropine and scopolamine detection in contaminated millet with Datura seeds) with HPLC-MS/MS. Now, I want to calculate LOD and LOQ. I know it...

07 February 2017 4,458 3 View

Anucleated, nucleated platelets and amebocyte?

08 September 2016 4,265 1 View

Has anyone experience with Pk probabilities method to assess the performance of a depth of anaesthesia monitor (i.e. BIS, Entropy)?

Is it possible to use Pk to compare the performance of a monitor in different sample groups (i.e. different ages groups)? I understand it is possible to use Pk probabilities to compare the...

04 May 2016 2,033 3 View

Any recommendation of statistical test ?

My research project is looking at the levels of anaesthetic in artery (ug/ml), vein (ug/ml) and oxygenator anaesthetic gas level (%).Theoretically, all the 3 compartments should be the same under...

13 December 2015 7,988 7 View

Any recommendation of statistical test ?

My research project is looking at the levels of anaesthetic in artery (ug/ml), vein (ug/ml) and oxygenator anaesthetic gas level (%).Theoretically, all the 3 compartments should be the same under...

11 December 2015 4,416 8 View