I am working on hydrophobic drug. DMSO is used as a solvent, when OD is taken it give me larger value. what is the best possible way to get appropriate value and nullify DMSO OD. Regards Nabeela Jabeen
To cut off the optical density (OD) values of DMSO in a sample, you can follow these steps:
By subtracting the OD of the blank solution, you can effectively remove the influence of DMSO on the measured OD values in your sample. This allows you to focus on the specific effects of the other components present in your sample.
It's important to note that this method assumes that DMSO does not interfere with the measurement at the specific wavelength you are using. Additionally, make sure to validate and verify this approach for your specific experiment and instrument setup.
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