What could happen if I stop a PCR reaction to add a component I missed out during the mastermix prep?

Let's say I forgot to add the polymerase enzyme and I only realize this after the run has gone half way, could I stop the reaction to include the pol...would I get amplification if I reprogram...

13 September 2019 3,211 3 View

Can heparin tubes be used to collect blood samples for Plasmodium falciparum microscopy?

I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.

23 April 2019 8,803 4 View

How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

17 April 2019 2,270 4 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

19 June 2018 4,721 2 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

24 September 2017 1,928 2 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

24 July 2016 507 5 View

Why is the concentration of ammonia in my reactor is decreasing while nitrite is increasing?

I am trying to establish anammox bacteria to coexist with ammonia oxidizers. Can someone explain to me why the concentration of ammonia in my reactor is decreasing while nitrite is increasing...

19 January 2015 9,816 7 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View