What is the dispersion relation in polycrystalline graphene?

Hi, We all know the dispersion relation for an ideal graphene in hexagonal lattice. Since graphene is polycrystalline by nature, assuming a perfect graphene crystals with average size La, nm...

24 January 2021 4,747 2 View

Can we consider that the earth is a prison for humans?

We can direct this question to astrophysics scientists, theologians, philosophers, scientists thought and civilizations! why? Astronomy shows that the universe is very wide and the distances...

29 July 2019 421 29 View

Any work done of removal of low levels of PFOS from drinking water using RO membranes?

Any work done of removal of low levels of PFOS from drinking water using RO membranes? All the 5 papers i found were testing high levels - not typical - I am looking to see the efficiency of RO...

11 April 2019 6,281 7 View

90% Confidence Interval Acceptable?

In most of social sciences research, 95% confidence interval is taken. My question is: If the research model is complex then can someone take 90% CI instead of 95%?

16 March 2018 7,092 9 View

What kind of experiment help in understanding the metabolic pathway in plants?

In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...

18 September 2016 396 3 View

In XRD of ZnO, Why the intensity of the (201) peak is more than the intensity of the common (002) peak?

16 May 2016 2,111 7 View

How can I compute the photometric transformations between two images?

22 December 2015 8,166 4 View

Is it bad if the strongest folding Tm (secondary structure) of my primers are closely similar to that of my primers' Tm?

Would the PCR reaction be hindered if my primers can form secondary structures at a temperature that is close to the primers' melting temperature? Since the temperature difference is small, the...

29 October 2014 6,381 11 View

Should I calculate the Tm for my primers before adding in the 5' restriction sites or after?

Am I supposed to first design my primers to get the closest matching Tm and then add in the restriction sites afterwards, or are the restriction sites supposed to be added to my primers first...

19 October 2014 400 11 View

If I were to clone a gene for expression studies, should I clone the full length gene or just the CDS (with/without the start and stop codons)?

The prokaryotic gene I'm looking at has conserved DNA sequences/ sequence motifs, but do not inlcude the start and stop codons within them although the motifs are part of the CDS. So if I were to...

09 October 2014 6,840 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View