Can someone with experience in routinely performing thermofluor assay or thermal shift assay using Sypro Orange (ex/em 470/570) and Mx3005p instrument provide me with guidance in terms of volume/quantity of protein and setting up the experiment?
Please, check Niesen et al. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nature Protocols 2, 2212 - 2221 (2007)
Article The use of differential scanning fluorimetry to detect ligan...