How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 2,808 9 View

In SEM, how to interpret standardised regression weights and their significance?

I have a SEM model that I'm having difficulty interpreting. It has one direct effect with a Beta/standardised regression weight that appears to be high (0.80) and significant (p

31 December 2020 7,604 6 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...

26 December 2020 4,480 4 View

How do I interpret these correlations and p-values?

I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...

17 December 2020 9,825 4 View

Is it possible to fuse Deep Learning Net and Clustering Algotrithm?

What I did (unpublished yet) is extract several (e.g. 16) mask features within Mask R-CNN from multiple images, construct a distance metric to compare them to predict the image class. Each set of...

13 December 2020 9,504 2 View

Are there models trained+evaluated on multiple datasets?

Generalization of COVID-19 models is a very serious challenge. I'm trying to find a model (ct scans or x-rays) that was trained and evaluated on multiple datasets. It would be particular...

03 December 2020 8,863 4 View

Are there models trained on both x-ray and ct scans for COVID-19 prediction?

I've only come across models that were trained and evaluated on one of these data. I don't see why it would be a problem to do both, as there are many similar features.

03 December 2020 8,310 3 View

When examining a series with different numbers of episodes per series, should I treat it as single sample, use harmonic mean or some other approach?

I'm currently examining how the behaviours of fictional characters are explained to child audiences through attribution theory as part of a study into the psychology of how children learn about...

27 November 2020 8,229 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Does the UV radiation affect on fruits ?

I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...

24 January 2021 8,350 20 View

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days?

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days. Anyone know reason behind this strategy

18 January 2021 1,166 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

You can mention a contribution that science will achieve in this year 2021?

It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...

02 January 2021 8,300 16 View