If we differentiate several stem cells (such as ES and iPSC) toward one specific tissue (such as muscle), how can we compare the product of these inductions (differentiated cells) with normal mature muscle?
Is there any study about it for all tissues?
Is there any publication about the best efficient stem cells for specific differentiation?
Dear Dr Razavi
This is a presented and published article on this matter.
https://www.researchgate.net/publication/332353228_Choosing_the_Right_Source_for_Induction_of_Pluripotent_Stem_Cells_for_Cell_Therapy_purposes?_sg=2zmRvTxp911eDWHDghmWFclzetXGl6j4keFRfCR7LMzEa_D_dMB_f88WGeHf8ExYxJMYfoYO8TUsNTw6BEUSViF8J_1WaTJJVc41b5Lu.hC2-2xIFTUJTXlBoLY6IkDKs_-3lxBsHFtiWsdUN4UjdNwuUO3F_FyQpYbr1L7VLTNjSakeDsrL5sc3xylih_w
I think Bioinformatics analysis is a good start.
Best
Hi, interesting question...however, Are "differentiated muscels" functional? Are you talking about myoblasts or true multinucleated muscle cells, or satellite cells?
@Pierluigi Scerbo
I want to compare the differentiated cells with normal adult cells.
In case of muscle, it is the differences between induction of every stem cell sources toward muscle and myoblast.
Hi, I hope this could help you:
In NCBI, go to "GENE" section and choose your gene of interest related to "muscle".
As a exemple, I put "MYF5" Homo sapiens, this is the result:
https://www.ncbi.nlm.nih.gov/gene/4617
In this window, you can see the locus your gene of interest (in this case MYF5) in human genome.
If you go down, you can see a section "EXPRESSION" (just below the intron - exon structure of your gene of interest.
In "EXPRESSION" section, you have 4 different RNA-Seq from different adoult tissues:
- Tissue-specific circular RNA induction during human fetal development
- RNA sequencing of total RNA from 20 human tissues
- HPA RNA-seq normal tissues
- Illumina bodyMap2 transcriptome
In the exemple I'm using (MYF5), i choose the second:
- Project title: RNA sequencing of total RNA from 20 human tissues
- Description: RNA sequencing of total RNA from 20 human tissues
- BioProject: PRJNA280600
- Publication:PMID 25970244
- Analysis date: Mon Apr 2 15:22:22 2018
- In BioProject you have all raw data from human adult tissues, like muscle, you can down-load it and comapre with your transcriptome.
- Good Luck
- Pierluigi
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