I'm doing ab initio protein structure prediction and I would like to compare my predictions using CASP targets with those proposed by the groups involved in CASP competition but I need their measures like GDT, QCS, DFM and CoDM scores etc.
How Artificial Intelligence can change the way to write academic thesis under master and Pd.D. levels are affecting the originality and knowledge of future academics.
09 June 2024 1,156 9 View
Dumbphones are based on basic synchronous wireless communications rather than the VOIP used in smartphones apps, like WhatsApp....which is far cheaper. Do you think that advertisements for...
05 June 2024 1,103 1 View
Dear colleagues Can I apply biofloc system in earthen ponds?
17 May 2024 2,685 0 View
I use Zsim software to fit and analyze my EIS-obtained results. I follow the following procedure: Step 1: I make another copy "save as" of the two columns Excel file of Z' and Z'' to *.txt...
27 April 2024 4,376 3 View
Compared to policy-based Deep RL algorithms, does this category of algorithms have a lower exploration efficiency ? I found this information in the following article: "Optimal energy management...
04 April 2024 225 1 View
What is the mathematical relationship or correlation between specific heat capacity and temperature of water?
22 February 2024 6,498 2 View
What is the most suitable vehicle that could be used to dilute bergamot oil?
13 February 2024 9,480 1 View
Hello colleagues, I want to determine which kit is better for extracting DNA from insects in bird feces, with the purpose of conducting barcoding later on. So far, we have been using a modified...
07 February 2024 2,601 1 View
I am making a research on artificial intelligence and fundamental rights. I would like to have some information about the topic in countries in the caribbean. Please, let me know if you have some...
17 January 2024 1,345 0 View
Hi everybody. I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation. For...
16 January 2024 8,821 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View