May I use Minimal important difference (MID) in non-normal distribution?

MID is based on Standard deviation, consequently, I should not use it in non-normal distribution. Am I right? If yes, which test may replace MID?

13 December 2020 7,573 3 View

How to formulate interview questions for Turnover Intention?

Currently, I want to gather preliminary data about turnover intentions among employee of a company using interview method. My biggest concern is with the corona pandemic, possibly each of the...

14 May 2020 768 5 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

Validation study: Can I join two samples from different regions of the same country?

I applied the same questionnaire in two samples (150 and 60 participants) from different regions from Brazil. I would like to join those samples, but I can not do measurement invariance (because...

10 March 2020 3,715 7 View

How can I check which constraints are violated (Concert - Cplex Studio 12.10 - C++)?

Currently, I am working on the implementation of a formulation for an optimization problem, at the moment I already have the MIP formulation implemented in C++ using Cplex studio 12.10 with the...

26 January 2020 7,887 1 View

Which is the best way to assess internal consistency in a non-normal data?

The sample coefficient alpha is affected by leptokurtic true score distributions, or skewed and/or kurtotic error score distributions. Therfore, I would like to found a alternative to Cronbach's...

05 March 2019 6,757 6 View

No title

31 October 2017 867 1 View

Using the Shiller PE Ratio (Cyclically Adjusted Price to Earnings - CAPE) on capital structure theory (trade-off, pecking order and market timing)?

Shiller PE Ratio seems to fit well as a proxy to test the Market Tiiming Theory along with the Market-to-Book (M/B). Since the M/B measures the managers 'perception of the company in the market in...

12 January 2017 4,941 2 View

How to reduce an Ester to aldehyde with no alcohol formation?

I'm using a procedure that uses morpholine and DIBAL-H. First I make the complex intermediate of the aluminum (1eq of DIBAL) with the morpholine (1eq) by stirring them for 3 hours. Then, I add my...

31 August 2016 5,531 21 View

How can I distinguish irreversible inhibition from noncompetitive inhibition since both have lower Vmax and unchanged Km?

I have come accross this tricky question and could not find a suitable explanation anywhere. Since both abovementioned types of inhibition show the same pattern, how can I make sure which one I am...

28 May 2014 7,575 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View