Hi,
I have been using bac-to-bac system for protein production using Sf9 and/or Tni cells. I am just curious to know if it is possible to construct baculovirus based plasmids?
Any suggestion is appreciated.
Thanks a lot!
Seema
For protocol see e.g.
https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-expression/insect-protein-expression/bac-to-bac-baculovirus-expression-system.html?gclid=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE&ef_id=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE:G:s&s_kwcid=AL!3652!3!389478895081!p!!g!!baculovirus
I hope it helps!
Thanks a lot Andreas and Roger!
To be more precise I have been looking for a method to prepare the construct. I have come across the following paper which is of great help.
Article Engineering the transposition-based baculovirus expression v...
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