Is there any protocol available to construct baculovirus based plasmids?

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Andreas Eisenreich

For protocol see e.g.

https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-expression/insect-protein-expression/bac-to-bac-baculovirus-expression-system.html?gclid=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE&ef_id=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE:G:s&s_kwcid=AL!3652!3!389478895081!p!!g!!baculovirus

Seema Nath

Thanks a lot Andreas and Roger!

To be more precise I have been looking for a method to prepare the construct. I have come across the following paper which is of great help.

Article Engineering the transposition-based baculovirus expression v...