Seema Nath

5 Questions 13 Answers 0 Followers

Questions related from Seema Nath

What’s your biggest equipment constraint when purifying proteins in bulk?

Following the series, the next puzzle is about equipment bottlenecks. Sometimes, the biggest challenge in scaling up protein purification isn’t the protein - it’s the gear. When you’re purifying...

last month 9,296 0 View

What is the biggest challenge you have faced when purifying proteins in bulk??

When working on protein purification, I’ve noticed something interesting: Scaling from a small test prep to bulk quantities isn’t just “more of the same.” It’s a whole new game with unexpected...

last month 3,226 3 View

Probable reasons of high background in Ni-NTA elution of secreted protein?

Hello !! I am trying to purify a secreted protein from Expi293 cells and was expecting a cleaner Ni-NTA elution profile unlike what I got (attached picture). Inspite of OD260/280 being >1 -...

19 November 2023 3,261 5 View

Are ExpiSf9 cells exclusive for suspension culture?

Hi All, It is recommended by Thermo Scientific to used ExpiSf9 cells as suspension culture. I am wondering if anyone has experience with other than suspension ExpiSF9 cell culture to produce...

13 February 2020 4,627 3 View

Is there any protocol available to construct baculovirus based plasmids?

Hi, I have been using bac-to-bac system for protein production using Sf9 and/or Tni cells. I am just curious to know if it is possible to construct baculovirus based plasmids? Any suggestion is...

27 December 2019 4,797 3 View