Seema Nath

3 Questions 12 Answers 0 Followers

Questions related from Seema Nath

Probable reasons of high background in Ni-NTA elution of secreted protein?

Hello !! I am trying to purify a secreted protein from Expi293 cells and was expecting a cleaner Ni-NTA elution profile unlike what I got (attached picture). Inspite of OD260/280 being >1 -...

19 November 2023 3,192 5 View

Are ExpiSf9 cells exclusive for suspension culture?

Hi All, It is recommended by Thermo Scientific to used ExpiSf9 cells as suspension culture. I am wondering if anyone has experience with other than suspension ExpiSF9 cell culture to produce...

13 February 2020 4,580 3 View

Is there any protocol available to construct baculovirus based plasmids?

Hi, I have been using bac-to-bac system for protein production using Sf9 and/or Tni cells. I am just curious to know if it is possible to construct baculovirus based plasmids? Any suggestion is...

27 December 2019 4,746 3 View