How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

What are the causes of hyphae granulation?

Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...

05 February 2021 5,523 4 View

What is the critical deficiency limit in soil for Mn in wheat crop ? in terms of DTPA extractable Mn?

critical range for Mn in soils is 2.5-4.5 ppm for all plants as an average, but what is the exact amount of DTPA extractable Mn in soil for wheat crop

04 February 2021 4,008 4 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

How to prepare wheat grain sample for amino acid analysis through UV-HPLC ?

how to prepare wheat grain sample for amino acid analysis through UV-HPLC, and also amino acid standards for HPLC , I will be very very thank full.

10 December 2020 1,207 3 View

How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

Calculation of dilution factor for fumonisin extracted rice cultured with fusarium species?

hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...

29 November 2020 1,831 15 View

Is Coefficient of Variability (CV) positively correlated with root mean squared error (RMSE) in every case??

My data for fungicide treated replication shows higher RMSE and R-square but less CV compared to the untreated replication for a regression between vegetation indices and yield. How correct is it?...

23 November 2020 2,079 6 View

Learning to discriminate mycorrhizal from non mycorrhizal fungi for McGonigle method. Can anyone recommend a handbook or manual for this?

To assess root colonization by AMF, I want to use the grid-line intersect method (Giovannetti & Mosse, 1980) or even the magnified intersection method (McGonigle et al., 1990). I understand the...

23 November 2020 5,338 8 View