You can store PCR product till one week in Room Temperature, For one or two month kept in 4 and for longer till one 1 year store at -20 degree centigrade.

I prefer to use 10mM Tris, pH 8.0 to store my PCR products. The tris helps stop the solution from becoming acidic (cause by CO2 being absorbed from the air)store PCR product at -20degree centigrade. I have found that stored in 10mM Tris the PCR products fridge for six months.

If you know you have a single product and have used a PCR cleanup kit or if you have gel extracted the product then most kits use the 10mM Tris pH 8.0 buffer used above that 90% of people I have come across throw it in the fridge at 4 degrees pretty much until they want it. However H2O is also ok fine too. I normally clone within a short time (within 2 weeks) but on the other hand I have been given products that have sat around for months at 4 degrees and it has still been fine. If you are paranoid then snap freeze it and leave at -20 but its more to unclog your fridge space than anything else.

You can store your pcr products at -20 deg C for about two months or at 4 deg C for 4 to 5 days. .

Hi, Kavion,

do you mean simple PCR, not RT-PCR?

In that case, as other people mention, you can store amplification product at 4 X for a while (1 week) or at -20 for mucg longer time. Thereafter just run it on the gel. Good luck!

Extract, speen, froze. Good for about a week, depends what kind of genes you target.

As the answers before already mentioned, there should not be a major problem to store PCR products; but what I can not really understand: why do you want NOT TO ANALYZE the result of your PCR immediately. I would suggest to collect the probes first, then perform the PCR and analyze it. Why wait?

They keep fine in a fridge for a while. I actually sequenced off of 6 month old mtDNA PCR product once. if you only have access to a low number comb or gel rig just make multiple "short" strips of gels and put them all on. As long as you don't overrun the gels you can do a lot in a little time.

@Taras: RT-PCR= Reverse Transcriptase PCR! qPCR= Real Time PCR (quantitativ)

You can store PCR product till one week in Room Temperature, For one or two month kept in 4 and for longer till one 1 year store at -20 degree centigrade.

Hi- according to my direct experience, you just need to freeze them around -20 °C or below and you'll be good for years ... !

Hi Kavin,

it doesn't really matter how you store PCR assays. There is hardly any reaction in biochemistry that has been boiled and thus freed of enzymes other than the polymerase itself than a PCR. Personally, I don't even cool PCRs down below room temperature after the last amplification cycle and I store them in the fridge if I intend to use them during the subsequent week. Freezing conservers the amplificates more or less indefinitely, at least for practical purposes. Only the water will dry away. We had never difficulties to clone ampification products after storing them for a year or so. Don't worry, in the absence of degradative enzymes, and at reasonable pH, DNA is a stable compound.

Best wishes - Johannes.

Since it is still in buffer, even room temperature is fine if you want to store for few days to a couple of month. Nothing had happend, in my case. But generally people store at -20. Reason i dont know ?

If you´re not sure when you´ll analyze the samples further just freeze them at -20°C and check them whenever you want.

I too agree with most of the answers that freezing them at -20 is sufficient to use them for a year or more.

I personally store them for short term storage at 4 degrees celcius, otherwise, i store at -20 degrees for longer period.

I am working on it and will update you soon......... All is well

Since the original question is over two years old, I guess, the problem could be solved in any way. Any update?

You can't do anything wrong (essentially). Cool it, freeze it; don't do anything if you wish. There is hardly any assay in the lab that has been boiled as often as a PCR. So just don't worry.

But if we store pcr products mixed with dye. Then what will be the effect of dye on amplified products. Will it improves its storage or decreases its storage period.

@Aejaz Dar: no effect to be expected (as long as your dye is not contaminated with nasty activities),

I agree with Ton that Huu

Do these procedures work for storing PCR product that will be used for a ligation into a plasmid, and then for transformation into competent bacterial cells?  cDNA is used as the template.  

Okey, may I have a query in here...

After storing amplified DNA, shall I add the dye and run on gel directly, or I have to something before, i.e. vortexing

Mention the chemical name of dye.

The answer r really good,,, more useful to me....

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