Sohrab Boozarpour

2 Questions 7 Answers 0 Followers

Questions related from Sohrab Boozarpour

How do cell lines arise from primary cell culture ?

I want to get cell lines from Mouse Primary Fibroblasts. Is there anyone who has experience in this field? Can anyone recommend a paper or book that can help me?

09 September 2013 1,934 21 View

Is polyA signal necessary for gene expression in lentiviral vectors?

I want to construct a lentiviral vector with Puromycine resistance gene. Is polyA signal necessary for expression of puroR? What happens, If we use this gene without polyA signal sequence?

02 February 2012 5,246 9 View