Does anyone have a protocol for betalain quantification by spectroscopic method from amaranthus spp.? - FAQS.TIPS

Kavin Soni @Kavin_Soni

03 March 2015 1 2K Report

phytochemistry

Does anyone have a protocol for betalain quantification by spectroscopic method from amaranthus spp.?

Ram Nath Mishra

The following article may help you.

Betalains in red and yellow varieties of the Andean tuber crop ulluco (Ullucus tuberosus)

J Svenson, BM Smallfield, NI Joyce… - Journal of agricultural …, 2008 - ACS Publications

... The extensive research by the Stintzing and Corke groups have revealed the betalain composition

of a ... purple pitaya, and cactus pear, and the list of identified betalains has reached ... Betaxanthins

and betacyanins were monitored and quantified at 470 and 538 nm, respectively. ...

Badges
Science topic

More Kavin Soni's questions See All

Does anyone have standard protocol for extraction of betalain pigment from different parts of amaranth plant?

I have extracted betalain pigment from sample. But after this step, for purification point of view, I am using aqueous two phase system method. Still have not been able to purify betalain easily.

03 April 2015 4,691 5 View

Which are the best methods for anti nutritional factor analysis in plants? Can anyone send me a link if possible?

Food & nutrition

01 February 2014 2,591 6 View

Can anyone suggest me any good methods for lysine, tryptophan & antinutritional factor analysis from maize?

Nutritional analysis.

31 December 2013 7,728 2 View

I want to determine total protein content from groundnut milk products like curd, paneer, cheese etc.

I want a sample preparation method for lowry & biuret method for protein quantification from curd, cheese, paneer that I prepared from ground nut milk. Kjeldahl apparatus is not present in working...

08 September 2013 4,609 4 View

Is there any procedure to store PCR products?

I am planning to do PCR for 100 different seed varieties so it is not possible for me to check PCR products on agarose gel in short period of time so I am seeking protocols to store pcr products.

07 August 2013 912 27 View

Similar questions and discussions

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View