Aristo Vojdani

There should be no problem having IgG and IgM tests in the same plate, but, preferably not in the same well. Note, that IgM values may be diminished when the levels of IgG in the same specimen are high. This can be circumvented by either (a) IgG stripping or (b) by using an IgM capture as the first step.

Stjepan Krešimir Kračun

Antoni Iborra

In my project, I am planning to develop an ELISA with the following goals

1. It should work on multiple marsupial species ( At least should work for about 5 common species)

2. Should able to pick up both IgG and IgM antibodies ( No previous studies have detected IgM, acute Toxoplasmosis in some marsupial species is very severe, so identification of IgM would be beneficial in early diagnosis)

It would be great if I can detect both IgG and IgM in the same well, so by a single serum sample, I can detect both anti-Toxoplasma IgM and IgG.

One of the main problems I am having to date is about secondary antibodies, as in human studies( like anti-human IgG , Anti-human IgM ) in wildlife studies, there are no species-specific secondary/detecting antibodies to differentiate IgG or IgM.

This is the only product available for marsupials ( Rabbit anti-Kangaroo Whole Serum Antibody)

https://www.bethyl.com/product/A140-105

But still, it would not differentiate between IgG or IgM, and also as it is " whole serum antibody" would bind with any other proteins too.

Thank you very much and highly appreciate your ideas.

Best Regards,

Tharaka