Tharaka Liyanage

10 Questions 12 Answers 0 Followers

Questions related from Tharaka Liyanage

Tips for using ABTS peroxidase in ELISA?

If anyone has used ABTS peroxidase in ELISA as the substrate, how long do you incubate before you stop/read the plates? I am going to develop an ELISA and plan to use ABTS peroxidase and aim to...

01 June 2023 1,407 4 View

Thoughts on using protein L in indirect ELISA to detect total antibody response?

I am planning to develop a multispecies (for marsupials) indirect ELISA and looking for a suitable antibody binding reagent. To my knowledge Protein A/G and protein L seem to be good candidates,...

02 September 2021 5,354 3 View

What Ct value is good in Taq Man probe based q PCR to make a cut off value to determine the positive or negative status of the samples?

Hello everyone, I am very new to q PCR and I am running a Taq Man probe-based q PCR assay to detect Toxoplasma gondii DNA in tissue samples of animals. I have generated a standard curve using 8...

15 April 2021 6,155 10 View

What would be the best method to get the q PCR standard curve for Toxoplasma gondii ?

It would great if anyone can share the experience whether you have used genomic DNA from cultures, or used cloning technique,s or used g Blocks to make the standard curve for T. gondii

05 March 2021 1,747 2 View

Can TaqMan® probes works with "Rotor-Gene Q" qPCR machine ?

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

02 March 2021 5,645 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

02 March 2021 8,920 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

I am looking forward to detect toxoplasma exposure in animals using Modified Agglutination Test (MAT), It seems the bioMérieux Toxoplasma MAT kit has discontinued its production. Can anyone...

19 January 2021 5,584 2 View

What is the purpose of internal control and extraction control in q PCR procedure?

Is extraction control also add to the qPCR mixture with the sample or does it run separately? Can anyone please explain their importance and how to use them in q PCR protocol? Thank you.!

24 November 2020 503 3 View

Can anyone explain the difference between recombinant proteins vs recombinant chimeric proteins?

Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests

29 September 2020 5,433 3 View

Is there any possibility to detect both IgG and IgM antibodies in the same ELISA plate?

I am looking forward to developing an ELISA which can catch both anti-Toxoplasma IgG and IgM antibodies in Marsupials. Has anyone have any thoughts about detecting two different antibodies ( IgG...

25 July 2020 4,342 3 View