I am looking for phytochemical screening protocols which is describe the methods for Extraction, Isolation,and Identification and analysis of Bioactive Compounds from Plant Extracts and advice for applying correct solvent and methods of extraction specially for Tobacco (Nicotiana tabacum L.) .So far only one book published from Jeffrey B. Harborne.I am looking specifically for bioactive compounds from tobacco leaf and protocols for Extraction, Isolation,and Identification and analysis of saponins, cardiotonic glycosides, alkaloids, terpenoids, lipids, volatile compounds, and polyphenols (flavonoids, xanthones,coumarins, naphthoquinones, anthraquinones, proanthocyanidins, etc.).Thank you for your kind support.
Hello I will be happy to help you; my doctoral thesis topic to focus on the identification of secondary metabolites in plants. I invite you to see my researchgate page, I publish my thesis in which you will find all the protocol you will need. Thank you and good luck.
Hii Ali Vafaei
you may get help regarding extraction protocol for triterpenoids from our published article (on my research gate page).
For all the other desired bio-active compounds, u may get a lot of protocols from google scholar.
Try these books: Trease and Evans pharmacognosy; or Jean Bruneton pharmacognosy, phytochemistry and medicinal plants.
There are different protocols, you can refer research papers of Phytochemicals from Google search
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