I am looking for phytochemical screening protocols which is describe the methods for Extraction, Isolation,and Identification and analysis of Bioactive Compounds from Plant Extracts and advice for applying correct solvent and methods of extraction specially for Tobacco (Nicotiana tabacum L.) .So far only one book published from Jeffrey B. Harborne.I am looking specifically for bioactive compounds from tobacco leaf and protocols for Extraction, Isolation,and Identification and analysis of saponins, cardiotonic glycosides, alkaloids, terpenoids, lipids, volatile compounds, and polyphenols (flavonoids, xanthones,coumarins, naphthoquinones, anthraquinones, proanthocyanidins, etc.).Thank you for your kind support.