I have strated lateral flow assay and the components are from clonegene. results are faint not clear...sometimes test line is coming sometimes control line is coming ..what should be the reason? Is there any paper availavle from LFA who have used clonegene components for my reference?
Héctor Osorio-Vega
Try drying the nc for longer. Are you using the proppike thater antigen conjugate for this?
The conjugate looks a bit dark, i would re check the conjugation conditions. The line should not look l
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