I saw some research groups using vacuum pump to seal the PDMS layer with the glass, while in this method you need to have according design patterns on the PDMS layer first. Below is an example I saw previously by using this method: http://www.nature.com/srep/2013/130731/srep02329/full/srep02329.html

My research group published some covalent chemical means to do this. Please see Ouellet et al., Langmuir 07/2010; 26(14):11609-14 or email me for a reprint.  

You can use simple thermal bonding method to seal PDMS to glass. Make sure both surfaces are flat and clean. Just put these two pieces together with gentle pressure, then bake in oven at 80 oC for over 1 hour.

We use this method to bond microfluidics devices, which can hold pressure at least to 10 Psi.

It's also possible with UV/ozone treatement.

You can use various ways to bond PDMS to glass:

1. try piranha (3:1 conc H2SO4: conc H2O2) treating the glass @ 80C for 2 hours, rinse it thoroughly dry it and then press the clean PDMS onto the glass. Other way is to even treat PDMS with piranha solution. 

2. You can even use corona discharge system.

3. Also you can try off ration system, wherein you can make PDMS (1:5 and 1:20) concentration and bond both the layers just by curing at 80 degree for 1 hour. (this system may work if you have option of using thin PDMS layer at the bottom instead of glass.

All the best. 

I don't know if you need to have a glass surface. If it's just to give stiffness to the device, you can spin-coat a thin layer of PDMS on top of the glass and use the ration system to get a permanent bonding to the bulk PDMS.

It is even possible to simple clamp the PDMS block to the glass mechanically, but be sure to use thicker glass ((0,28 - 0,32 mm, still suitable for confocal microscopy). You can find a photograph of the device we use in the ESI of this article: http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/c4lc00460d#!divAbstract

http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/c4lc00460d#!divAbstract

Article Controllable electrofusion of lipid vesicles: Initiation and...

Hello..

I have to bond pdms device with gold coated glass slide.

Can anyone suggest me the best method??

I have tried plasma treatment method but after inserting the fluid, bond is break.

Did you find any other way apart from plasma, to bond the PDMS chip to glass slide?

The more simple method is placed PDMS and glass together and keep it in plasma cleaning at atmospheric pressure for about 30 seconds.

You can use the curing agent to bond the pdms with glass.

Just keep some drops of curing agent on the glass substrate and keep the pdms on this . Apply temperature around 70deg and little pressure .

Maxime Harnois Can you please tell me how long I need to treat with UVO-cleaner. I am using 3 mins but it can't hold the very little hand pressure with syringe.