Are there any ways to merge the outputs of Siamese and U Net at the end, which are trained by the same datasets?

I am training the same datasets on different networks. I want to know if there are any possibilities to combine the outputs of these two networks.

27 August 2020 6,090 1 View

How do I calculate the recirculation length behind a bluff body in FLUENT?

Hi, I am currently working on a simulation of external flow over a semi-circular cylinder with the curved surface facing the flow. I would like to calculate the length of the recirculation region...

26 March 2017 6,779 4 View

How do I solve the divergence problem?

helloI am doing thermal and hydraulic study of flow past perforated fins using turbulent K-Omega SST model in ANSYS Fluent. After few iterations, my Energy equation start to Diverge. Please...

24 March 2017 3,279 6 View

How can I create a monitor for velocity at a point in FLUENT?

Hi, I am working on a transient simulation of the flow over a square prism and I want to create a monitor for the z-velocity at a particular point in the wake region (flow is in the +ve x...

16 February 2017 1,442 6 View

Is it possible to compare the surface net towed copepods abundances with the Niskin collected (100 L) subsurface copepod abundances ?

30 March 2016 2,807 7 View

How can I calculate L-asparaginase enzyme unit?

Hi All, I want to calculate the L-asparaginase enzyme activity. I ain't sure which formula should I use. If I use the standard one [1 unit equalling the amount of L-asparaginase that liberates 1...

06 March 2016 778 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View