Is there any other way to make the electrophoresed gel to react with gelatin substrate?

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Peter Pediaditakis

The short answer is "No".

If you have nothing to clear than you have nothing to see. I can not think of a way to move gelatin into get with out moving protease out.

Luc Nelles

one way is to overlay with a gelatin slab and let the proteins diffuse into the overlay

http://what-when-how.com/molecular-biology/overlay-assay-enzyme-zymography-of-plasminogen-activators-and-inhibitors-molecular-biology/

Israa AL-Kadmy

Dear Yogi

No, you don't do that

and don't see any think in the gel