Hi Mohamed,

There are a number of different techniques available to measure nanoparticle size other than DLS

NTA- https://www.malvernpanalytical.com/en/products/product-range/nanosight-range/nanosight-ns300

RMM- https://www.malvernpanalytical.com/en/products/product-range/archimedes

TDA- https://www.malvernpanalytical.com/en/products/product-range/viscosizer-td

SEM

TEM

AFM

SAXS

TRPS

UV Vis

Note that the "sizes" measured by these techniques are not all the same. DLS, NTA and TDA all measure hydrodynamic size whereas electron microscopy measures the size of a hard electron dense core of the particles.

As a matter of interest what is your aversion to the NanoBrook? There are other DLS solutions available.

Dear Dr ali,

The machine which you mentioned is really a very nice machine to determine nano size of the particles (particle size analyzer) -Nano Brook.

Apart from this specially for particle size XRD and UV vis can be used. In both the process you need to process the obtained data on standard scherrer equation (xrd)and Tauc plot (from UV data).

Other method can also be used based.on the availability.

Hi Mohamed,

There are a number of different techniques available to measure nanoparticle size other than DLS

NTA- https://www.malvernpanalytical.com/en/products/product-range/nanosight-range/nanosight-ns300

RMM- https://www.malvernpanalytical.com/en/products/product-range/archimedes

TDA- https://www.malvernpanalytical.com/en/products/product-range/viscosizer-td

SEM

TEM

AFM

SAXS

TRPS

UV Vis

Note that the "sizes" measured by these techniques are not all the same. DLS, NTA and TDA all measure hydrodynamic size whereas electron microscopy measures the size of a hard electron dense core of the particles.

As a matter of interest what is your aversion to the NanoBrook? There are other DLS solutions available.

Most widely used as well as convenient way to measure the size of nanoparticles/micelles is DLS.

Although TEM is also a very useful tool to measure the size of particles/vesicles/micelles and fiber/nano-rod width.

You can try any of them. All the best.

Small-Angle X-ray Scattering technique can give you quantitative information about the size, shape and surface of nanoparticles, in the case of micelles, for example, the inner and outer diameter.

You can use DDM (Differential Dynamic Microscopy) is an alternative method to DLS. With DDM you can also get video particle tracking.

You can follow above mentioned techniques as per availability near your locality.

Оne of Methods to meazurre the size of namoparticl is using of its levitatation

SEM, DLS are poor method to measure the particle size. AFM and HRTEM are the most suitable method to measure the accurate particle size.

@ Chandra You must have a reason or reasons for your answer. Please elaborate.

@D. Yu. Skubov- Do you have a reference for this approach that you could share?

@ Dr Alan ,

The DLS method does not provide much size distribution but rather mean size and estimate of polydispersity of the suspension. A small quantity of small size particles can easily be hidden in a much larger quantity of large size particles.

In SEM characterization, If samples are magnetic then it become difficult to resolve the SEM image of nanosamples . Beause for highly magnetic samples , the intensity of electron beam of SEM may not be sufficient to provide the required resolution to make estimates of nanoparticle sizes. This fact makes it impossible to make accurate estimates of particle size distribution of SEM image of nanosamples. So HR-TEM is suggested for it.

Hello Mohamed,

extending the above methods there is a family of Feld Flow Fractionation methods (FFF). The particle size specific methods are simple FFF and asymmetric flow FFF (AFFFF).

@ Alok XRDLB does not produce particle size but crystallite size (although these may be close for single nanoparticles). There are plenty of discussions on Research Gate on this topic.

@ Chandra I ask only one question. Are particles sold or processed on the basis of numbers or on the basis of volumes or masses?

I could ask a second question. How many particles are needed to specify the x99 point in a distribution to 1% standard error?

Dear Mohamed,

I see a lot of methods being suggested here in this forum. But, since I work on micelles, I know the practical difficulties. Micelles are usually either liquids or polymeric globules. XRD is meant only for solid particles. XRD will not work on micelle as the basic principle of XRD is diffraction from surface electrons of the sample, but the micelles do not have free electrons on the surface.

DLS is the only method (as far as I know) how you can calculate its size.

Hi

Please find attached file. I think it will be very helpful for you.

Regards

Dear Jenan,

Thanks

Mohamed G Ali, have you calculated the particle size, and if yes then by which method you did it.