10X MOPS buffer is used for formaldehyde gel electrophoresis of total RNA.
Is there any other alternative for this buffer that can be used in formaldehyde gel electrophoresis?
Yes. Hepes/triethanolamine and Tricine/triethanolamine are two buffer systems that have been used in place of MOPS.
Yes. Hepes/triethanolamine and Tricine/triethanolamine are two buffer systems that have been used in place of MOPS.
To add to what Syed A Ali is saying,
You can find all the details in this article:
http://www.ncbi.nlm.nih.gov/pubmed/23800830
OR
You can do the glyoxal denaturation + BPTE gels which also gives really nice gels but takes much longer to execute from A to Z (see Molecular Cloning a laboratory manual).
Don't use formaldehyde....
You can use form amide solution in agarose gel (0.8%) to seperate Total RNA. You will find three types of RNA (mRNA , rRNA and tRNA) and 3 intact clear bands ....
Total RNA from prokaryots
http://www.spandidos-publications.com/article_images/etm/7/4/ETM-07-04-0901-g01.jpg,
Total RNA from Eukaryots
https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcS7SuWwSHH0_tDKDIU4LfIvbF2K_mRv2ydnePmVEc00hkNyh1rJF
For ex:
That is 4:1 ratio, 40ml of 1X TBE or TAE and 10ml of formamide solution.
Take 40 ml buffer and add 0.4g agarose, melt it then cool for hand tolerable temperature, finally add 10ml of formamide solution mix well gently then, etbr fr ur conc.
We are all on the same page here -- we are talking about gels for Northern Blots and RNA analysis. Here's a related piece of information that I think is very important in terms of minimizing exposure to formaldehyde. Did you know that formaldehyde can be eliminated from the gel itself, and only added to the sample buffer before loading the gel? After I read this paper decades ago, I use the technique and the gels look the same as when formaldehyde is added to the whole gel.
See: LIU,Y.C. and Y. C. CHOU, 1990. Formaldehyde in formaldehyde/ agarose gel may be eliminated without affecting the electrophoretic separation of RNA molecules. Biotechniques, vol. 9 pp.558-560.
Sorry, I do not have the reference to upload.
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