Mutation is known to be permanent alteration of the nucleotide sequence of the genome of an organism,
Could there be any benefits from it?
The population genetics of mutations: good, bad and indifferent
Article The population genetics of mutations: Good, bad and indifferent
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...
18 February 2021 3,811 7 View
Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...
04 February 2021 2,047 4 View
Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...
03 February 2021 2,154 8 View
Hi I want to set up a virus neutralisation assay such as PRNT or FRNT at my lab (BSL2), but im uncertain of the bio safety requirements that has to be fulfilled in order to work with...
01 February 2021 5,626 3 View
I'm trying to use LINK11 to apply a force as if it was a cable pulling a structure twowards a point in ANSYS APDL. When I solve the model, the deformation of the overall structure is far too...
30 January 2021 7,919 1 View
We have been having clotting issues with our Cytoflex S from Beckman Coulter. Back in December 2020, we had to have the tubing system and sample needles replaced, but we are beginning to observe...
28 January 2021 6,897 2 View
I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...
27 January 2021 6,852 3 View
Most of us use tooth paste more times a day. The empty tube we throw away. I wondered how empty the tube really is. This does not sound being Research? I began to collect empty tubes...
19 January 2021 808 3 View
We all use face masks many hours a day. I wonder what are your items you like to be improved. We have the opportunity to write an application for support to a sponsor. Your answers will help us a...
18 January 2021 1,311 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...
18 February 2021 371 4 View
When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...
09 February 2021 5,423 1 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...
07 February 2021 3,123 8 View
I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.
04 February 2021 8,872 2 View
Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...
03 February 2021 6,408 2 View
I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...
01 February 2021 5,216 4 View
I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...
31 January 2021 355 2 View