Hi, I am going to start labwork for a research in a week. This requires drawing blood from patients, and then aliquotting plasma & serum into 500microlitre eppendorfs after seperation and then storing at -80 degrees celsius. (I will be using BD vacutainer plasma and serum separator tubes) However due to unavoidable circumstances (lab closing indefinitely) I will have to postpone the aliquotting part. My questions are;
a) is there any method to keep plasma and serum in those PST and SST tubes itself (refrigerated or frozen) for sometime?
b) Or is it okay to take plasma and serum into separate EDTA tubes and store frozen at -20 degrees celcius (or a higher temperature), so that i can thaw them again to aliquot later?
Really appreciate any advices..
Stability varies from parameter to parameter.
you should not store/ refrigerate the whole blood samples in PST and SST tubes or EDTA tubes.
best way is to separate the plasma / serum into plain eppendorf tubes and store at-20 degrees celcius .
But again stability of the required parameter need to be checked.
Fresh frozen plasma to store in approved freezers at less than -30°C. It is thawed just before use (a process which takes up to 30 minutes) and once thawed, must be infused within 24 hours if kept at 4°C (or 4 hours if kept at room temperature)
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