Umeshi Karunadasa

5 Questions 2 Answers 0 Followers

Questions related from Umeshi Karunadasa

Transient BBB Disruption in In Vitro Models Using Mannitol or Other Agents?

In animal models, hyperosmolar agents like mannitol transiently disrupt the blood-brain barrier (BBB). Does anyone know of similar methods for BBB disruption in in vitro models, particularly in...

17 February 2025 6,715 1 View

Passage effect on HBEC-5i cell line?

Hi everyone, I have got a vial of HBEC-5i (Human Brain Endothelial Cells) for studies on blood brain barrier. The company delivered it frozen at passage 18. I have some questions about this; 1. Is...

23 September 2024 8,801 3 View

Co-culture of SH-SY5Y cells and astrocytes?

Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...

02 July 2024 331 2 View

DNA extraction from blood for Conventional PCR - how long can we keep the EDTA tubes in room temperature?

I am planning to obtain human blood samples in EDTA tubes which will be followed by column-based DNA extraction (DNEasy extraction kit). These samples will be subjected to FlaB nested PCR to check...

15 December 2021 2,244 2 View

Is there any method to temporarily store human plasma and serum, for some days prior to aliquotting into smaller volumes?

Hi, I am going to start labwork for a research in a week. This requires drawing blood from patients, and then aliquotting plasma & serum into 500microlitre eppendorfs after seperation and then...

03 September 2019 7,064 2 View