Umeshi Karunadasa

2 Questions 1 Answers 0 Followers

Questions related from Umeshi Karunadasa

Co-culture of SH-SY5Y cells and astrocytes?

Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...

02 July 2024 243 2 View

DNA extraction from blood for Conventional PCR - how long can we keep the EDTA tubes in room temperature?

I am planning to obtain human blood samples in EDTA tubes which will be followed by column-based DNA extraction (DNEasy extraction kit). These samples will be subjected to FlaB nested PCR to check...

15 December 2021 2,217 2 View