Is there any method to find out if Aflatoxin B1+BSA conjugate is degraded ? I have a stock of 1mg/ml stored in -20 degree and would like to cross check if anything is wrong with the stock.
Hi,
You can try to run a TLC plate or HPLC against a standard. Anyway, Aflatoxins as far as I know are extremely stable and heat resistant. Even autoclaving procedures do not destroy it. So, I think that putting it in -20C for storage + BSA will keep it for a very long time. I previously used an aflatoxin standard for a TLC run which was almost 10-15 yrs old stocked in the freezer (consider freeze-thaw during power failures in our lab) and it still works.
Hope this helps!
Are you concerned about the aflatoxin or the conjugate? As indicated above the aflatoxin itself is quite stable. The protein (BSA) and (stability of) the conjugate might be a different story.
Sir @Kris Gerard Alvarez thank you for the reply. I have run a TLC and there was a difference in the Toxin alone and the BSA conjugate.
But as C.A. (Kees) Kan asked I m concerned about aflatoxin+BSA conjugate.
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