How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

How to clean reverse phase C18 column?

I prepared a column (reverse phase C18) for Medium Pressure Liquid Chromatography (MPLC). I injected my crude extract 5 times & now the column has become pale yellowish. My crude extract...

01 November 2020 5,276 4 View

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp?

Can anyone recommend a method of isolating terpenes (including sesquiterpenes) from dried hemp? The idea is to separate the cannabinoids fraction from the terpenic fraction. We will use...

29 September 2020 1,267 3 View

How to perform in silico peptide cyclization reactions by ChemAxon Reactor?

24 April 2020 9,398 1 View

Short Peptide synthesis, Fmoc-SPPS wth citrullin, why can it show a different mass?

I tried Fmoc-SPPS with short cyclicPeptides (12 aa length, Brom-Acetyl at the start for cyclization), for the first time containing citrollin. Many of the petides show a main Peak at the MALDI MS...

31 March 2020 3,986 3 View

How imide is formed by reaction between 4-nitrophthalic anhydride and melamine by using one mole of both?

I have performed this reaction many times by using acetic acid as a solvent bt no cyclization occurs to form imide.

05 March 2020 6,051 3 View

Which materials/metals have specific adsorbing abilities towards Limonene?

I am working on a project regarding the Terpene adsorbing capabilities of different metals/materials. I wasn't able to find adsorbing properties of any material specifically towards limonene. Can...

04 February 2020 6,034 3 View

Any fastest method avilable to isolate the terpenoids from the petroleum ether extract of plant source?

I want to isolate the compounds from petroleum ether extract of plant source. what is the new technique available? and I want to know the place where, the facility is available in India?

31 December 2019 5,721 2 View

For saturated hydrocarbons in the extracts, why only n-alkanes exist but steranes and terpenes are absent ?

All Ordovician limestones were extracted to get the extractions, and the saturated hydrocarbon of which had been analyzed using a SHIMADZU GC-2010Plus equipped with an FID. The results showed that...

11 December 2019 7,960 2 View