I used linalool standard for estimating total terpenoid content for my plant sample, different linalool concentration has been taken and finally the supernatant has been discarded, the red precipitate ring is treated with solvent. while discarding the supernatant either micro ml of precipitate been drawn or left with micro ml of supernatant, due to this i am unable to draw a standard curve and the values are non-linear . kindly help me out to find a better way for doing this experiment
I believe the best way to do it is through GC-FID analysis. You can use linalool as internal standard, as long as there isn't linalool in your sample. It is difficult to give a precise answer with the information provided. But if you have you plant extract (i.e.: an essential oil), you can dilute an aliquote in hexane and add the standard. Both extract and standard masses will be known, at the end of the chromatographic run you can relate the areas of the extract peaks to the standards.
I hope it helps.
Thank u for ur response Sir . But my work is to just estimate the total count of terpenoid using basic standard protocol. Now I switched to ursolic acid with some modified procedure for this estimation.
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