How to perform cys -cys cyclization of peptide (disulfide bond formation)?

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Timofey Kudryavcev

2,2'-Dipyridyldisulfide

Leonardo González Ceballos

Hi Ramkamal!

Follow this:

Procedure:

The peptide is dissolved in a solution of HOAc 5% in water (c(pep) = 0.2 mmol/L); pH is adjusted to 6 by adding ammonia solution; DMSO is added to obtain a percentage of about 20 %. The mixture is left to stir at rt. until the linear peptide dissapears.

Good luck!

Marco Sette

Hi Leonardo and Timofey, do you have a reference for that?

Marco