I dont think so, beause it need both

you should use the Gene runner programme.

Thanks Basil A. Abbas. Yes it needs both but the blast software often shows alignments of one strand.

If you blast genomic sequence you need to look into the annotation of your hits. To do this I prefer the ensembl.org site.

In case you blast transcribed sequences only the coding (sense/ nontemplate) strand is in the data base. The numbering will tell you whether your hits align with the reverse complement sequence.