Is there any method for extraction of outer membrane protein form gram negative bacteria without using ultracentrifuge

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Does Azospirillum has a role on phosphorus mobilization and uptake in spring maize? If yes, which strains?

In context of undergraduate research on PUE and azospirillum inoculation in maize.

20 March 2024 5,084 0 View

How to standardize methods for determination of drug residue in tissue by HPLC?

29 November 2023 4,159 1 View

How to calculate water storage in soil profile without lysimeter?

I have 5 cm, 8 cm, 10 cm, 18 cm, 20 cm volumetric water content data from TDR sensor that records every 15min. I want to know ΔS at (0-30 cm) to calculate ET by water balance method. I changed...

30 March 2021 933 5 View

What is the suitable method to find out the DC conductivity of glass and hence activation energy for the conduction, experimentally?

I am preparing oxide glasses (Silicon-phosphate) by melting and quenching method. I want to measure DC conductivity of the glass obtained after annealing. I am trying with Van der Pauw Four probe...

27 August 2020 1,192 3 View

How can I connect Matlab with Digsilent?

I want to know how could it be possible to make a connection between Matlab and Digsilent. for example, the power system is modeled in Digsilent and an optimal power flow problem is intended to be...

07 June 2020 8,449 3 View

What is the best way to store tomato flowers to extract pollen from them later? What is the most efficient method of testing tomato pollen viability?

I am planning to do pollen viability and germination of tomato plants under heat stress condition. Tradition methods to test pollen viability are time consuming. So, I'm curious if there are any...

20 October 2019 3,959 5 View

How far we have reached in the development of drought tolerant rice and is it possible to reduce the methane emission with this rice field?

It has been know that the rice field contributes to the significant amount of methane emission to the atmoshere. So, I wonder how far we have reached in the development of drought tolerant rice...

01 July 2019 1,548 5 View

How to do molecular dynamic simulation?

I want to carryout molecular dynamic simulation of some amphiphilic molecules to study their assembling behavior. My particular interest is in the lamellar bilayer arrangements. Someone, please...

16 May 2019 10,183 7 View

How can we measure sugar, protein, vit C, nitrate and protein content in leafy vegetables?

Composition analysis

19 December 2018 7,102 3 View

What next of 'resilience' in the coming decade?

A new concept has evolved almost every decade over the last 6 decades such as relief/response operations, community based disaster preparedness (CBDP), community based disaster management (CBDM),...

26 September 2018 5,621 10 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View