Is there any method for extraction of outer membrane protein form gram negative bacteria without using ultracentrifuge

More Samikshya Kafle's questions See All

What is the suitable method to find out the DC conductivity of glass and hence activation energy for the conduction, experimentally?

I am preparing oxide glasses (Silicon-phosphate) by melting and quenching method. I want to measure DC conductivity of the glass obtained after annealing. I am trying with Van der Pauw Four probe...

27 August 2020 922 3 View

How can I connect Matlab with Digsilent?

I want to know how could it be possible to make a connection between Matlab and Digsilent. for example, the power system is modeled in Digsilent and an optimal power flow problem is intended to be...

07 June 2020 8,137 3 View

How to do molecular dynamic simulation?

I want to carryout molecular dynamic simulation of some amphiphilic molecules to study their assembling behavior. My particular interest is in the lamellar bilayer arrangements. Someone, please...

16 May 2019 9,997 7 View

Why do not we see mRNA as a distinct band on RNA gel?

we extract RNA and run on 1.2% agarose gel but could not see the distinct band of mRNA on the RNA gel.

21 April 2016 5,112 4 View

Is there any portable instrument to measure CaCl2 concentration (0.1-2%) in water?

Can we directly relate pH, EC and alkalinity with CaCl2 concentration?

07 April 2015 4,923 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View