Ayushi Gupta

5 Questions 6 Answers 0 Followers

Questions related from Ayushi Gupta

Can anyone suggest me the DAPI concentration (less than 300nM) that works good with IHC?

I am using 300nM concentration of DAPI for 5 minutes in IHC (paraffin fixed tissue) and it is getting overstained.

27 February 2025 1,662 2 View

Is there any method by which we can test the anticancer property of the compound, without using any cells or cell line?

I have searched for the methods where I do not require any cell or cell line, but I could not find it. I want to check the anticancer property of the given compound (protein) without using cells....

22 March 2024 10,056 3 View

How can we graft cancer cells on cam of egg for in-ovo analysis?

I want to graft the cancer cells i.e, MCF7 on cam of egg. To do this I need to have some scaffold. In a paper i read about matrigel, is there any other alternative of matrigel or can we graft the...

04 January 2024 8,895 6 View

How to do protein protein docking?

I have two proteins for which i want to check the interaction. I have searched and a server named Cluspro appeared. Is it okay to use a server for protein protein docking? If yes then how will i...

08 December 2023 2,349 2 View

Can I use EMEM complete media with 10% FBS for MRC-5 cell line?

In which culture medium MRC-5 cell line will have good growth?

25 September 2023 9,466 2 View