Is there any human differential expression data for PDE5 inhibitors (human cell/cell line/tissue microarray or NGS)?

Alexander Zhavoronkov @Alexander_Zhavoronkov

12 December 2014 1 6K Report

Do you know of any gene expression data sets for different PDE5 inhibitors in cell lines/tissues in humans?

We are specifically looking for data on:

avanafil

sildenafil

tadalafil

vardenafil

udenafil

Go J Yoshida

Cell/ tissue-dependent expression pattern of phosphodiesterase 5A, cGMP-specific, abbreviated as PDE5, is shown in the following URL and attached file.

http://www.genecards.org/cgi-bin/carddisp.pl?gene=PDE5A&search=ffe4627d14100692b7193d288b408402

Badges
Science topic

More Alexander Zhavoronkov's questions See All

Is there a good overview of mathematical approaches to measuring human altruism?

Can you recommend a few papers on quantifying the outcomes of altruistic behavior that go beyond net QALY? Not talking about Price equation or Hamilton's rule here.

03 April 2016 6,237 7 View

Are there any good tools or services for scoring clinical trials?

Clinical trials are extremely complex when it comes to interpreting outcomes data. Are there any tools that: 1. help find what clin trials failed and what succeeded (i.e. even tracking phase...

06 July 2015 6,719 0 View

What is the best platform for classifying PubMed output?

I need to enter a term into PubMed and classify the output by journal type, paper type, impact factor of the journal and cluster the output by disease ontology. What are the best online tools to...

06 July 2015 8,154 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View