Hi,

I have a synonymous variant library of a protein, and it has hundreds of variants. They are cloned in the Flp-In T-REx expression vector to work with the Flp-In system. We have worked with this library to measure the protein levels using the Flp-In HEK293 cell line and it has always worked. Right now I would like to transfect this library into other human cell lines and unfortunately, these new cells do not have the Flp-In T-REx landing pad and it would require a lot of work to generate them.

I wanted to ask if there is any other high throughput method to measure the protein levels of these variants in human cells.

Thanks a lot.