Is there any high throughput agar plate assay to screen a bacterial MUTANT hyperproducing the rhamnolipid (biosurfactant) from the wild type?

18 July 2014 1 9K Report

We can differentiate a hyperproducer from wild type by CTAB and haemolysis but these assays are not promising if we are screening from large number of colonies. So I need to know methods for selection of general/specific mutants.

Thavasi Renga Thavasi

Try 24 wells agar plate that will reduce the number of plates. Also try drop collapse assay that will help to screen more samples and it is a very easy method too.

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