Hello everyone,

I am working on hCB1 in CHO cells and I got low response of HU210 

I homogenised the cells by using buffer (50mM, 2mM EDTA and 5mM MgCl2) then wash three times with high centrifuge at 30,000 at 10min.

In terms of binding assay, I used the same buffer at 25C at pH 7 for 90 min (I tried 30C and 37C but there is no response). In fact, this condition works well with rat brain but not with CHO-hCB1.

Can anyone give me an advice in binding assay generally? 

Regards,